Analysis Note

ControlCONTROL ANTIGEN: Included free of charge with the antibody is 120 µg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For positive control, in Western blotting, use 20 ng protein per lane. For negative control, preincubate 3-5 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196 detects level of Potassium Channel MaxiK & has been published & validated for use in WB.

Western blot: 1-1.5 μg/mL (1:200:1:300) using ECL

Dilutions should be made using a carrier protein such as BSA (1-3%). Typical bands detected are ~125kDa in rat membrane preparations. Excellent methods given in Ling, et al (2003) {http://jp.physoc.org/cgi/content/full/553/1/65}

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: C-terminal sequence SHSSHSSQ

A purified fusion protein (GST)1 and a C-terminal part (residues 1097-1196) of mouse Slo (mSlo) alpha subunit of BKCa channel.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 µL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Specificity

Corresponds to the C-terminus of the longer form of BKCa channel a subunit and overlaps by >40 residues with a shorter form.

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.